Saturday, 8th February 25th Annual Lorne Proteomics Symposium 2020

Steve Tate, PhD – Manager Software Applications Research Group – “Enabling ultra-fast proteomics with deep sample coverage”

Jérémy Potriquet, PhD – Field Application Scientist & OMICS Market Development – “ Evaluation of the use of in silico libraries in DIA experiments”

• Fitting big science on a small page - Martin Krzywinski
• Trans-omic characterisation of stem cell networks - Pengyi Yang
• The Human Protein Atlas - Implications for Human Biology and Precision Medicine - Cecilia Lindskog
•  Phosphomatics: A knowledge-based approach to investigating high-throughput phosphoproteomics data - Michael G Leeming
• Delving deeper into the proteome with an improved and highly parallelised feature detection pipeline for the Bruker timsTOF Pro - Daryl Wilding-McBride

• Fidelity of protein synthesis is required for energy production - Aleksandra Filipovska
• Addressing unmet clinical diagnostic needs with proteomics - Michelle M Hill
• Global proteomic and phosphoproteomic profiling to identify proteins and pathways that regulate cell survival in hypoxic conditions - Keshava K. Datta
• Analysis of FFPE tissues to investigate lymph node protein expression in patients with leukemia - Lauren A Thurgood
• Simultaneous mass spectrometry imaging of multiple neuropeptides in the brain and alterations induced by experimental Parkinsonism and L-DOPA therapy - Per E. Andren
• Reference proteome-based subtyping of liver cancer - J. Eugene Lee
• Ciliary proteome from motility-defective multiciliated cells - Taejoon Kwon

• Mini-malting and mini-mashing methods for early stage validation of new barley varieties and malt accreditation and quality control of beer brewing - Christopher H Caboche
• The role of upstream phosphorylation in the regulation of histone methylation - Ryan Separovich
• Revealing the proteome of brain derived exosomes isolated from human amyotrophic lateral sclerosis post-mortem tissues - Natasha Vassileff
• Development of a primary cell proficient phosphoproteomic workflow - Rune H Larsen
• Studying uncharacterised non-structural proteins of Rabbit haemorrhagic disease virus  - Elena Smertina
• Mapping the lysosomal transporter proteome of osteoclasts uncovers new regulators of bone homeostasis - Amy BP Ribet

• Proteomic Characterisation of poor sperm chromatin compaction suggests nuclear retention - Jacob Netherton
• Bacterial death phase initiation by targeted membrane lipid modification - Shuai Nie
• Defining the interactome of the immunity protein GccH - what can it tell us about glycocin F induced bacteriostasis?   - Gillian E Norris
• Using open source data for peptide spectral library generation with data independent analysis label free quantification (DIA-LFQ) for plasma biomarker discovery - Matthew O'Rourke
• Unravelling the biological mechanisms of Acute Lymphoblastic Leukemia (ALL) drug-resistance with integrated multi-glycoconjugate analysis - Tiago Oliveira
• TANDEM ION MOBILITY COUPLED WITH MASS SPECTROMETRY FOR GAS PHASE UNFOLDING STUDIES - Heather Patsiouras
• Technical Reproducibility and Analytical Precision for a Multi-omic Study utilising Data Independent Strategies with CCS libraries - Robert S Plumb
• Turnover optimized short nanoLC gradients on a tims equipped QTOF for high throughput and deep proteome measurements - Adam Rainczuk
• Identification of Epidermal Growth Factor Receptor Variant III (EGFR VIII) Expression in Meningioma Indian Patients - Rashmi Rana
• A statistical approach to rank differentially expressed proteins using confidence bound on effect size. - Anup D Shah
• Molecular signature associated with lymph node metastasis identified from minimally invasive FFPE colon cancer - Pascal Steffen
• Wheat pan-proteomics: Unifying data-independent LC-MS proteome measurements across diverse genetic backgrounds for trait prediction - Sally Stockwell
• Coordinated post-transcriptional and post-translational regulation through stage transition of Giardia duodenalis - Qiao Su
• Mass spectrometry-based proteome screening of two narrow-leafed lupin cultivars - Arineh Tahmasian
• Detecting Proteomic Differences Between Nevi And Early Melanoma Using SWATH-MS - Rachel Teh
• Protein-based human identification - Rebecca Tidy
• Mediator Lipidomics: Towards Comprehensive Metabolic Profiling of Eicosanoids and Related Fatty Acids - Atsuhiko 'Ash' Toyama
• Unravelling the molecular contributions to collagen higher order structure - Danielle R Visser
• Phosphomatics:  A free web based tool for the analysis of phosphoproteomic peptide lists - Nicholas A Williamson
• Surviving ER stress in a mouse model of ulcerative colitis - Richard Wilson
• Diapasef: Toward The Ideal Mass Analyzer With Data-Independent Acquisition And Parallel Accumulation – Serial Fragmentation - Steve Wilson
• Evaluation of a tims-Q-TOF instrument for targeted proteomics - Steve Wilson
• Achieving optimal study designs for large-scale proteomics - Merridee A Wouters
• A Maleimide-functionalized Tetraphenylethene for visualization and quantitation of  Unfolded Proteins in Cells - Shouxiang Zhang
• Improved middle-down characterization of antibodies using multiple ion activation techniques and Proton Transfer Charge Reduction on an Orbitrap Eclipse mass spectrometer - Steve Binos
• An Orbitrap Eclipse Tribrid mass spectrometer with real time search enhances multiplexed proteome coverage and quantitation accuracy. - Bruno Madio

Mass spectrometry (MS)-based proteomics has become a powerful technology for the identification and quantification of thousands of proteins. However, the coverage of complete proteomes is still very challenging due to the limited speed, sensitivity and resolution of current mass spectrometers.

The timsTOF Pro uses the Parallel Accumulation Serial Fragmentation (PASEF) acquisition method to provide extremely high speed and sensitivity to reach new depths in shotgun proteomics and phosphoproteomics, using low sample amounts.

We are privileged to have two international speakers who will present some of their cutting edge results from the latest ion mobility enabled platform


Prof. Matthias Trost - Professor of Proteomics Institute for Cell and Molecular Biosciences, Faculty of Medical Sciences, Newcastle University, UK

Using the timsTOF Pro to study ubiquitylation in innate immunity

Dr. Ben Collins - School of Biological Sciences, Queens University, Belfast, UK

Data-independent acquisition combined with parallel accumulation – serial fragmentation combined (diaPASEF): Bottom-up proteomics with increased ion usage

• Defining the "mucinome": enzyme toolkit for enrichment and analysis of mucin-domain glycoproteins - Stacy Malaker
• Tools and methods for probing the biology of tryptophan C-mannosylation - Ethan Goddard-Borger
• Granule-Specific N-Glycosylation and Chlorination Activity of Neutrophil Myeloperoxidase - Morten Thaysen-Andersen
• Immunopeptidomic analysis reveals that deamidated HLA-bound peptides arise predominantly from deglycosylated precursors - Shutao Mei
• Synthetic Glycopeptides assisted large scale glycoproteomics from sample preparation to data analysis - Kathirvel Alagesan

• Proteomics Combined with Human Cardiac Organoids to Find New Mechanisms Driving Maturation and Regeneration - James Hudson
• Characterisation of a putative new metabolic hormone in human plasma - Mark Larance
• Strategies to enrich low-molecular weight proteins for proteomic analysis - Parthiban Periasamy
• Rapid separation and identification of beer spoilage bacteria by inertial microfluidics and MALDI-TOF mass spectrometry - Brooke A Dilmetz
• Deglycosylation of glycoproteins with trifluoromethanesulfonic acid (TFMS): unravelling the chemistry allows extension of the protocol to the analysis of released glycan chains using standard proteomics methods - Paul D Veith