Here, we present ‘Phosphomatics’ – a new web-based tool for interrogating possible upstream kinases for phosphorylated substrates observed in phosphoproteomics experiments. Phosphomatics takes an input list of phosphopeptides, which can include fold change information, and automatically searches databases of known substrate-kinase relationships for information on any known upstream kinase/s that are recorded as being responsible for specific phosphorylation events. Thus Phosphomatics can rapidly identify possible upstream kinases that may be active in an experimental dataset. Phosphomatics additionally maps the phosphoproteins and the possible kinases on to KEGG signalling pathways, connects the user to the primary literature sources, can identify similar substrate peptides in the dataset, and has an inbuilt text-mining feature for rapid literature searches. Phosphomatics primarily utilises data from the SIGNOR database of causal relationships and then connects other databases and web tools such as UNIPROT, PubMed, Protein Atlas, and iTextMine.
Phosphomatics is a free and user-friendly web resource for biologists to rapidly interrogate their phosphoproteomics datasets.
Phosphomatics is freely available via the internet at: www.phosphomatics.com